
American Foulbrood (AFB) is caused by a bacterium called Paenibacillus larvae. Oxytetracycline is used to treat infections but strains of oxytetracycline resistant P. larvae have been identified in some countries.
Different concentrations of HiveAlive were evaluated for their inhibitory potential using agar-dilution assays and 96-well microdilution assays. A concentration of 0.25% HiveAlive in agar was sufficient at inhibiting growth on solid media. In liquid media, growth appeared to be inhibited at 0.625%. Further tests are required to define the minimum inhibitory concentration and to assess if this inhibitory action can be replicated in larvae.